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Light microscopy techniques

 

The Centre for Cellular Imaging facility can offer advanced techniques that employs fluorescence to take the step into the sub-resolution domain for light microscopy:

Fluorescence Correlation Spectroscopy (FCS)

Measures diffusion coefficients and concentrations of fluorescent molecules in solution and in living cells by monitoring fluorescence intensity fluctuations in a single spot.

Fluorescence Recovery After Photobleaching (FRAP)

Studies diffusion in living cells by bleaching a small region in a specimen and monitoring the fluorescence recovery with a time series.

Fluorescence Resonance Energy Transfer (FRET)

Studies molecular interactions by exciting a donor fluorophore, which transfers the energy to an acceptor fluorophore if they are in close proximity.

GFP Recognition After Bleaching (GRAB)

Correlative microscopy between light and electron microscopy. Bleach your fluorophore with light and get contrast in the EM image at the exact location of the fluorophore.

Laser Microdissection and Pressure Catapulting (LMPC)

Non-contact sampling, especially for RNA, DNA and protein studies but also for cloning/live cell culture purposes. Microdissect a part of your specimen with a laser and catapult it into a collection device.

For protein studies the masspec analysis can be performed by the Proteomics facility, and the laser microdissection can then be offered as a CCI service for shorter projects. See LMPC followed by LS-MS/MS analysis for more information.

 

 

Contact Information

Centre for Cellular Imaging

See "Contact - The CCI People",

Visitors and delivery

Visiting address
Medicinaregatan 7A
413 90 Gothenburg

Delivery address 
Medicinaregatan 1G
413 90 Gothenburg

Post address
Centre for Cellular Imaging
University of Gothenburg
Box 413
40530 Gothenburg
Sweden

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Page Manager: CCI web manager|Last update: 5/22/2018
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