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LSM 710 NLO

 

Whether it’s intravital imaging, long-term observation of developmental processes, or high-resolution 3D imaging, the LSM 710 NLO from Carl Zeiss delivers true-to-detail and high-contrast images. The outstanding sensitivity of the systems is combined with innovative techniques to suppress laser light excitation. Improved non-descanned detectors (NDD) with extraordinary light collection efficiency and the GaAsP technology also guarantee excellent imaging results in thick tissue samples and living animals. The system is optically perfect for the entire wavelength range from 350 to 1100 mm. Specimen illumination controlled with single-pixel accuracy.

 

LSM 710 NLO System Characteristics


Axio Examiner– the upright microscope platform for intravital microscopy; it offers a lot of space for the specimen and adjusts to every requirement. To make the sample area easily accessible and to provide optimal viewing, the optical axis of the stand is right in front. With a sample area up to 10 cm, the Axio Examiner is especially suited for dealing with living animals. The components are controlled via the separate TFT Remote Control Panel or via controls positioned at the very front of the stand.


The Scan Module – Core of the LSM 710
The motorized collimators, the scanners, the precisely positionable pinhole and the highly sensitive detectors are arranged to provide optimum specimen illumination and efficient collection of the emitted light. The scanning module (Fig. 1) has 34 simultaneous confocal fluorescence channels (34-channel QUASAR) with highly sensitive low dark noise PMTs; spectral detection range freely selectable (resolution down to 3 nm); up to 10 individual, adjustable digital gains. You can simultaneously use up to ten markers, with excellent image quality and least possible time expenditure. The scan resolution from 4 x 1 up to 6144 × 6144 pixels; also for multiple channels. Scanning speed up to 12.5 frames/sec with 256 × 256 pixels; 8 frames/sec with 512 × 512 pixels (max. 77 frames/sec 512 × 32); min 0.38 ms for a line of 512 pixels; up to 2619 lines per second. Master-pinhole pre-adjusted in size and position, individually variable for multi-tracking and short wavelengths (e.g. 405 nm). Beam path: exchangeable TwinGate main beamsplitter with up to 50 combinations of excitation wavelengths and outstanding laser light suppression; low-loss spectral separation with Recycling Loop for the internal detection 

The Beam Path
The unique design allows for the best possible combination of light efficiency, flexibility, ease of maintenance and retrofitting, and compactness.

Fig. 1 LSM 710 NLO Scan Module (from Carl Zeiss)

Objectives
High transmission to the fluorescence signal and the excitation light minimizes performance losses in either direction. A high numerical aperture objective creates a smaller focus with higher photon density for efficient excitation, at the same time maximizing the collection also of scattered signal light, in particular. All the objectives are corrected for chromatic aberrations (Apochromat); this correction facilitates the superposition of excitation planes of different wavelengths.

Water Plan-Apochromat 20x/1.0 DIC 75mm (WD=1.9 mm), ∞/0
Water Plan-Apochromat 20x/1.0 DIC 75mm (WD=1.9 mm),∞/0.17
Plan-Apochromat 10x/0.45 (WD= 2.1 mm), ∞/0.17
Plan-Apochromat 20x/0.8 (WD= 0.55 mm), ∞/0.17
Plan-Apochromat 40x/1.4 Oil DIC (WD= 0.13 mm), ∞/0.17

Epi-fluorescence illumination
Metal halide illumination source (Fig. 2), Illuminator HXP 120:
These light sources feature a high-performance arc discharge lamp housed in an elliptical reflector that focuses the output into a liquid light guide for delivery to the microscope optical train.


Filter set 49 DAPI
Filter set 43 Cy 3
Filter set 38 GFP
Filter set 43 Cy 5                

 


 

 

Fig.2 "Education in microsocopy and digital Imaging",  from Carl Zeiss.

 

Vis Laser Lines
Pigtail-coupled lasers with polarization preserving single-mode fibers; stabilized VIS-or direct modulation; 4 UV/VIS-laser directly mountable into the scanhead; Laser diode (405 nm) 25 mW; Ar-laser (458, 488, 514 nm) 25 mW; Laser diode (561nm); HeNe-laser (633 nm) 5 mW

 

Non-Descanned Detectors (NDDs)
Fluorescence light excited with the multiphoton laser originates from the focus only. Thus, no confocal pinhole is needed, and the signal can be projected directly from the specimen onto the detector. Our LSM 710 NLO has two non-descanned detection channels for reflection and two for transmission:
Filterset 1u green/blue (BP 500-550 / SP 485)
Filterset 2u red/green (BP 565-610 /BP 500-550)
 

GaAsP Non-Descanned Detector
The challenge of intra-vital imaging is the need to visualize very fine details in tissue that may be extremely light-scattering. The GaAsP Non Descanned Detector with its high efficiency detection and low dark noise is the ideal tool for such tasks. This detector not only increases the flexibility but provides an up to 2fold betters signal-to-noise ratio and correspondingly a higher sensitivity.
 

Multiphoton Laser
The InSight DeepSee is our ultrafast laser system that takes multiphoton imaging to new depths! The laser is more robust and fully automated, providing easy-to-use, hands-off operation, freeing users to focus on their critical research. the InSight DeepSee delivers nearly double the tuning range of existing ultrafast lasers and provides seamless access to long infrared wavelengths for deepest in vivo imaging: 680 nm to 1300 nm continuous tuning from a single source, short 100 fs pulse widths and highest peak power levels into the infrared where imaging penetration depth is maximized. Spectra-Physics integrated patented DeepSee, the industry standard dispersion pre-compensator, delivers the short pulses through a microscope to the sample for maximum fluorescence. InSight DeepSee also has exceptional beam pointing stability, beam quality and output power stability, making it ideal for microscopy. A dual wavelength with a second output beam complementing the main tuneable output is also available for advanced imaging techniques including uncaging, CARS imaging and multimodal imaging. This fully automated ultrafast laser can be seamlessly tuned with the click of a mouse to any wavelength within seconds at speeds over 50 nm/sec.
 

 

ZEN imaging software
ZEN microscope software leads you simply and quickly to the result. At all times you see which options the system is making available to you and which step is appropriate to take next. Use ZEN imaging software to achieve scientific success: nothing diverts you from your goal - to document your results and reproduce them at any time, more...

Page Manager: CCI web manager|Last update: 11/1/2017
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