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ELYRA PS.1 SIM/PAL-M LSM780

ELYRA PS.1 SIM/PAL-M LSM780 is a state-of-the-art inverted microscope designed for imaging in laser scanning confocal, super-resolution structured illumination (SR-SIM) and single-molecule localization (PAL-M) microscopy modes. The microscope frame is equipped with a dual-camera adapter that couples two electron-multiplying CCD cameras for imaging in two channels. A laser scanning module, the LSM 780, allows the specimen to be probed with point-scanning confocal microscopy in thin sections. The ELYRA module contains turnkey components that allow the operator to image a selected region of the specimen to gather PAL-M images for super-resolution analysis or structured illumination SIM. Finally, the microscope is equipped with software that enables image assembly and analysis for all three imaging modes.

ELYRA PS.1 SIM/PAL-M LSM780 System Characteristics

Laser scanning microscope LSM 780 - Challenging samples require more than just amplifying weak signals. You need a detector that’s capable of dealing with high signal dynamics, provides low noise and allows for short pixel times. To achieve such performance the LSM 780 is equipped with a 32-Channel GaAsP spectral detector actively cooled and photon counting with almost twice the Quantum Efficiency (45% QE typically). To complement this detector, two side PMTs expand the spectral working range and allow additional imaging of very strong signals for higher dynamics. The group of 32 2 detectors allows you to reproduce spectral measurements reliably and without deviation. Since this is a parallel spectral detection design, it offers simultaneous 34-channel readout in lambda mode. Up to 10 dyes can be acquired and separated at the same time. With the new GaAsP detectors, you get up to 2x better SNR for 2x faster acquisition.

 

 

Thanks to its excellent sensitivity and counting ability, the LSM 780 lets you tap into the power of integrated spectral Fluorescence Correlation Spectroscopy (FCS) to analyze single molecule dynamics. The LSM 780 is able to perform FCS analysis with the actively cooled, photon counting GaAsP detector. As a result of the spectral capability of the GaAsP detector, the dyes you choose can now be located anywhere in the whole color spectrum. That makes the LSM780 much more versatile. Up to 6 channels can be used in FCS mode, providing great flexibility in your stainings and samples. 

ELYRA SR-SIM and PALM illumination module
The module include a fully motorized SR-SIM imaging; five different grating frequencies for SR-SIM for optimal matching of illumination pattern to laser wavelength and objective lens; motorized exchange of gratings in multi-color SR-SIM; pattern rotation with adjustable number of angle steps (3 or 5 r otations).

The PALM illumination include a fully motorized total internal reflection (TIRF) illumination; simultaneous TIRF illumination with VIS and 405nm laser lines; individual triggering of lasers for synchronizing dye activation and illumination to camera read and transfer times; motorized TIRF angle adjustment; motorized TIRF field adjustment with three field size options.
 

Cameras
Camera for PAL-M, mounted to side port of microscope: Andor iXon 897 back-thinned EMCCD camera with a nominal quantum-yield higher than 90% and single photon detection capability that detects the generated fluorescence pattern of the individual molecules; 512 x 512 pixels; and 16 μm x 16 μm pixel size. The registered images can be analyzed on-line and can be computed to reconstruct an image of the specimen on a molecule-by-molecule basis.

Camera for SR-SIM, mounted to base port of microscope: Andor iXon 885 EMCCD camera with a large field-of-view, up to 75 x 75 μm in SR-SIM (with Plan-Apochromat 63x /1.4 oil immersion); 1004 x 1002 pixels; and a 8 x 8 μm pixel size. The registered images can be subsequently computed to generate an image of the specimen with a resolution that is up to twice the spatial resolution (axial and lateral) given by the classic diffraction limit in all three dimensions.

Lasers
LSM 780 laser module equipped with 4 Lasers:
Diode laser (405 nm, CW/pulsed) 30 mW
Argon-laser (458, 488, 514 nm) 35 mW
DPSS-laser (561 nm) 25 mW
HeNe-laser (633 nm) 5 mW

Super-resolution laser module equipped with 4 Lasers:
Diode laser 405 (50 mW)
Solid-state 488nm (100 mW)
Solid-state 561nm (100 mW)
Solid-state 642 nm (100mW)

Filters
Filter GFP mRFP Alexa 633 shift free Fluorescence (red/green) to ocular

Filter modules for super-resolution microscopy on a motorized turret:
-405 EF BP 420-480 / LP 750 SR, 405 Laser
-488 EF BP 495-550 / LP 750 SR, 488 Laser
-561 EF BP 570-620 / LP 750 SR, 561 Laser
-642 EF LP 655 SR, 642 Laser

Objective lenses
Plan-Apochromat 10x / 0.3 objective (LSM)
Plan-Apochromat 20x /0.8 objective (LSM)
Plan-Apochromat 40x /1.4 oil immersion objective (LSM)
C-Apochromat 40x/1.2 water immersion (LSM / FCS)
Plan-Apochromat 63x /1.4 oil immersion (SR-SIM / LSM)
C-Apochromat 63x/1.2 water immersion (SR-SIM / LSM)
Alpha “Plan-Apochromat” 100x /1.46 oil immersion (SR-SIM / PALM / TIRF)
Alpha “Plan-Apochromat” 100x/1.57 oil immersion (PALM / TIRF)

Page Manager: CCI web manager|Last update: 11/24/2015
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